Properties of New, Long-Wavelength, Voltage-sensitive Dyes in the Heart
نویسندگان
چکیده
منابع مشابه
Imaging activity of neuronal populations with new long-wavelength voltage-sensitive dyes.
We have assessed the utility of five new long-wavelength fluorescent voltage-sensitive dyes (VSD) for imaging the activity of populations of neurons in mouse brain slices. Although all the five were capable of detecting activity resulting from activation of the Schaffer collateral-CA1 pyramidal cell synapse, they differed significantly in their properties, most notably in the signal-to-noise ra...
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Long wavelength voltage-sensitive dyes (VSDs) called Pittsburgh (PGH) dyes were recently synthesized by coupling various heterocyclic groups to a styryl-thiophene intermediate forming extended, partially rigid chromophores. Unlike most styryl VSDs, dyes with a sulfonic acid anchor directly attached to the chromophore showed no solvatochromic absorption shifts. The limited water solubility of ma...
متن کاملUse of Voltage - Sensitive Dyes and Optical
-Understanding the spatio-temporal features of the information processing occurring in any complex neural structure requires the monitoring and analysis of the activity in populations of neurons. Electrophysiological and other mapping techniques have provided important insights into the function of neural circuits and neural populations in many systems. However, there remain limitations with th...
متن کاملPalette of fluorinated voltage-sensitive hemicyanine dyes.
Optical recording of membrane potential permits spatially resolved measurement of electrical activity in subcellular regions of single cells, which would be inaccessible to electrodes, and imaging of spatiotemporal patterns of action potential propagation in excitable tissues, such as the brain or heart. However, the available voltage-sensitive dyes (VSDs) are not always spectrally compatible w...
متن کاملPotassium Sensitive Optical Nanosensors Containing Voltage Sensitive Dyes.
lonophore-based ion-selective optical nanosensors have been explored for a number of years. Voltage sensitive dyes (VSDs) have been introduced into this type of sensors only very recently, forming a new class of analytical tools. Here, K(+)-sensitive nanospheres incorporating a lipophilic VSD were successfully fabricated and characterized. The nanosensors were readily delivered into the social ...
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ژورنال
عنوان ژورنال: Journal of Membrane Biology
سال: 2005
ISSN: 0022-2631,1432-1424
DOI: 10.1007/s00232-005-0826-8